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Real-Time PCR
High-Speed Real-Time PCR on Microchip
Polymerase chain reaction (PCR) is now often indispensable technique used in medical and biological research labs for a variety of applications including qualitative and quantitative nucleic acid analysis; DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and paternity testing); and the detection and diagnosis of infectious diseases.


   PCR analysis time (45 cycles) 20 minutes
    due to high thermocycling rate (1012 °C/s);
  Low sample and reagent consumption
    only 0.51.8 μl of 2x PCR master mix for 0.51.8 μl sample;
  Low detection limits
    15 DNA/RNA copies per microreactor;
  Microchips with ready-to-use immobilized PCR reagents;
    reduce users labor content;
  Qualitative and quantitative DNA/RNA analysis
    simultaneously in 2048 microreactors via 2 detector channels (FAM, SYBR Green / ROX);
  Minimizing contamination
    PCR is performed in a microchip isolated from environment;
  Real-time data monitoring
    current process settings (temperature, PCR cycle); analysis time; expected time to complete analysis; PCR curves parameters; plus/minus DNA analysis results;
  User friendly graphical interface;
  Automatic report generation;
  CFR 21 Part 11 complaint;
  Export results in industry standard data formats.
  Microchip-based real-time PCR analyzer for rapid qualitative and quantitative analysis of nucleic acids in various samples.
  Microchips for user-designed PCR reagents can be used with various reagents according to the user tasks. PCR mixtures of reagents and samples can be added into microreactors in the same way as in a test tube.

Disposable microchips in different microreactor formats and materials:
silicon microchips with 20, 24, 30 microreactors;  aluminium microchip with 30 microreactors.
Microchips with lyophilized reagents for the detection and diagnosis of infectious diseases; functional analysis of genes; the diagnosis of hereditary diseases. Microchips with lyophilized PCR reagents can be transported and stored at ambient temperature up to 6 months.
   **All you have to do is to add a sample with a buffer into the microreactors**
    Lumex has designed test systems for identification of  Salmonella spp and designed a microchip for determination of pathogen in chickens, feed and poultry products. On a single microchip 14 unknown samples maximally, positive and negative control samples can be analyzed.




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